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CDNA SYNTHESIS KIT
Product code – ODR23
RTase used in this kit is a thermostable mutant of M-MLV reverse transcriptase (RNase H-) which is a recombinant type and purified from E.coli. This mutant type of RNase enables more efficient cDNA synthesis due to more thermostable characteristic compare than wild type through the prevention of abnormal cDNA of secondary structural RNA under the temperature of 42ºC.
Kit Contents
| Description | Quantity | Storage |
|---|---|---|
| RTase(200U/μl) | 10000 U | -20°C |
| 2X Real-Time PCR Smart Mix
(Included 10mM dNTP mix) |
200μl | -20°C |
| 8 mM DTT | 500μl | -20°C |
| RNase free Water | 0.5ml | -20°C |
| Random hexamer or Oligo dT (2.8nmol) | Lyophilized | -20°C
Dissolve in 56μl nuclease free water before use. |